Questions and Methods
Biological control is achieved when an invasive plant's natural enemies (herbivores) are introduced to reduce the fitness of the targeted invasive plant. Schutzenhofer and Knight (2007) conducted an experiment to artificially examine how increased levels of herbivory on an invasive plant species, Lespedeza cuneata, impact the fitness (growth and seed set) of this species, i.e., would biological control have the potential to curb the growth rate of this invader. Their experiment was set up by first finding plants that were of different size classes: small (1 branched individuals), medium (2-5 branched individuals), large (6-10 branched individuals), and extra-large (>10 branched individuals). For each size class they had five different clipping treatments: control (no augmented herbivory), 20%, 40%, 60%, and 80% augmented herbivory (see image 1a &1b). The purpose of the clipping treatments was to augment herbivory. Clipping consisted of manually removing leaf tissue by hand to mimic a leaf-chewing herbivore (herbivorous biological control agent). As previous work has found that ambient levels of herbivory on this species are minimal, the researchers assumed that all leaves were equivalent with regards to original amounts of damage. For example, in the 20% augmented herbivory treatment, 20% of all tissue was manually removed and plants in this treatment were estimated to have a total amount of 20% "herbivory".
Results
At the end of the growing season they measured the amount of seed produced by all plants (seed set). They found that the treatments did not affect seed set of plants in any size class. Then, in the next year, they determined if the plants survived and whether or not the plants decreased or increased in size (to measure growth). The only size class in which clipping treatments affected growth and survivorship was the small size class, which is shown in Figure 1a and 1b.